Putrescine, DNA, RNA and protein contents in human uterine, breast and rectal cancer.
J Postgrad Med
; 2000 Jul-Sep; 46(3): 172-5
Article
in En
| IMSEAR
| ID: sea-117489
ABSTRACT
AIMS:
To find out the status of DNA, RNA and protein in human uterine, ovarian, breast and rectal carcinoma. MATERIAL ANDMETHODS:
In this prospective study, patients of age group between late thirties and late fifties suffering from uterine, ovarian, breast and rectal cancer were taken as subjects of the present study. The total number of cases studied for each cases was ten. Pieces of human carcinomatous tissues of above mentioned cases were taken along with surrounding normal tissues. From the tissue samples, putrescine is separated by the method of Herbst et al, DNA analysed by Diphenylamine method, RNA by Orcinol method and protein by Biuret method.RESULTS:
Tissue content of putrescine rises simultaneously with that of DNA, RNA and protein in carcinomatous growths as above in comparison to their respective adjacent normal tissue, the differences being statistically highly significant.CONCLUSIONS:
Increase in DNA, RNA and protein concentration may be a pre-requisite for increased synthesis of putrescine in carcinomatous tissue and thereby the concentration of other di- and poly-amines.
Full text:
1
Index:
IMSEAR
Main subject:
Ovarian Neoplasms
/
Rectal Neoplasms
/
Spectrophotometry
/
Uterine Neoplasms
/
Biopsy, Needle
/
Breast Neoplasms
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Female
/
Humans
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Male
/
DNA, Neoplasm
Type of study:
Diagnostic_studies
/
Observational_studies
Language:
En
Journal:
J Postgrad Med
Year:
2000
Type:
Article