A simple and cost effective liquid culture system for the micropropagation of two commercially important apple rootstocks.
Indian J Exp Biol
; 2014 Jul; 52(7): 748-754
Article
in En
| IMSEAR
| ID: sea-153755
ABSTRACT
The two commercially important apple rootstocks i.e., MM106 and B9 were micropropagated using a liquid culture system. Three different strengths of 0.8% agar solidified PGR free basal MS medium were first tested to optimize the culture media for both the rootstocks. Full strength medium (MS0) supported maximum in vitro growth, multiplication, rooting and survival under field conditions as opposed to quarter and half strength media. When three different volumes of liquid MS0 were tested, highest in vitro growth, multiplication, rooting and also survival under field conditions were achieved in 20 mL liquid MS0. The cost of one litre of liquid medium was also reduced by 8 times to Rs. 6.29 as compared to solid medium. The cost of 20 mL medium was further reduced to Rs. 0.125.
Key words
Full text:
1
Index:
IMSEAR
Main subject:
Plant Growth Regulators
/
Biotechnology
/
Cost-Benefit Analysis
/
Plant Roots
/
Culture Media
/
Malus
/
Culture Techniques
Type of study:
Health_economic_evaluation
Language:
En
Journal:
Indian J Exp Biol
Year:
2014
Type:
Article