Evaluation of ‘cattle’ and ‘Indian Bison’ type antigens of Mycobacterium avium subspecies paratuberculosis for diagnosis of Bovine Johne’s Disease using ‘indigenous ELISA’ and AGPT .
Indian J Exp Biol
;
2014 Dec; 52(12): 1182-1185
Article
in English
| IMSEAR
| ID: sea-153809
ABSTRACT
Two antigens (‘cattle’ type and ‘Indian Bison’ type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne’s disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Paratuberculosis
/
Cattle
/
Enzyme-Linked Immunosorbent Assay
/
Precipitin Tests
/
Cattle Diseases
/
Reproducibility of Results
/
Bison
/
Sensitivity and Specificity
/
Mycobacterium avium subsp. paratuberculosis
/
Host-Pathogen Interactions
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
2014
Type:
Article
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