DPPH radical scavenging activity and contents of H2O2, malondialdehyde and proline in determining salinity tolerance in chickpea seedlings.
Indian J Biochem Biophys
; 2014 Oct; 51(5): 407-415
Article
in En
| IMSEAR
| ID: sea-154272
ABSTRACT
The involvement of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and contents of H2O2, malondialdehyde (MDA) and proline was investigated in determining salinity tolerance among seedlings of thirty chickpea (Cicer arietinum L.) genotypes having different pedigrees. Chickpea genotypes, including cultivars and advanced lines were grown for 7 days under control and salt stress (50 mM NaCl) conditions. The genotypes showed differential response to salt stress in terms of growth, DPPH radical scavenging activity and contents of H2O2, MDA and proline in seedlings. On the basis of seedling growth, the genotypes having better performance under stress conditions had reduced levels of H2O2 and MDA contents, but increased levels of proline and DPPH radical scavenging activity. Stress tolerance index for these parameters was also determined. Agglomerative hierarchal clustering by Pearson correlation coefficient grouped the genotypes into two major clusters — MC I and MC II. MC II and A1-1 sub-cluster of MC-I comprised mainly of genotypes that showed higher stress resistance levels for the respective parameters in comparison to genotypes in other sub-clusters. Thus, it is possible to identify salt-tolerant genotypes on the basis of above parameters without a field trial.
Key words
Full text:
1
Index:
IMSEAR
Main subject:
Picrates
/
Biphenyl Compounds
/
Proline
/
Free Radical Scavengers
/
Reactive Oxygen Species
/
Oxidative Stress
/
Cicer
/
Seedlings
/
Salinity
/
Salt Tolerance
Language:
En
Journal:
Indian J Biochem Biophys
Year:
2014
Type:
Article