Cloning of Υ derived DNA sequences of bovine origin in Escherichia coli.

ABSTRACT

We have constructed a partial library of Υ chromosome derived DNA sequences of bovine origin in Escherichia coli. That, the recombinants arc Υ derived and Υ specific was ascertained by differential colony hybridization using male and female DNA probes. Out of 1000 recombinants analysed, 17 were found to be Υ derived as well as Υ specific and were of repetitive nature. Restriction analysis revealed that most of them had short DNA inserts