Specific staining of myo-inositol-1-phosphatase on polyacrylamide gels after electrophoresis.
J Biosci
; 1989 Sep; 14(3): 249-253
Article
in En
| IMSEAR
| ID: sea-160733
A sensitive staining method was developed to localise the activity of myoinositol- 1-phosphatase on Polyacrylamide gels after electrophoresis. The method can also be used for non-specific phosphatases as well as for those specific phosphatases acting upon inositol polyphosphates which are prime cellular second messengers. One or two nmol of phosphate is sufficient and less than 3 μg of purified protein will facilitate the localisation of phosphatase. If more phosphatases are present in the enzyme preparation, a combination of inhibitors can be used to suppress the activities of unwanted phosphatases and the use of specific substrates will facilitate the localisation of enzyme of interest.
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Language:
En
Journal:
J Biosci
Year:
1989
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Article