Cytokine generation in stored platelet concentrate: comparison of two methods of preparation.
Article
in En
| IMSEAR
| ID: sea-16224
ABSTRACT
BACKGROUND & OBJECTIVES:
Contaminating white blood cells (WBCs) in stored platelet concentrates (PC) are the main source of pro-inflammatory cytokines including interleukin-6 (IL-6), interleukin- 8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) that are implicated in transfusion reactions. We compared the levels of these cytokines in stored platelet preparations prepared by two methods. Effect of pre-storage leucofiltration on these cytokine levels was also studied.METHODS:
Twelve units of pooled PCs were prepared by platelet rich plasma (PRP) method and buffy-coat (BC) method each and stored for 5 days. IL-6, IL-8 and TNF-alpha levels were measured in platelet supernatants on day 0, 1, 3 and 5 of the storage using commercially available immunoassays. Pre-storage leucofiltration was done in 4-pooled units of PRP-PC and cytokine levels compared with unfiltered PCs.RESULTS:
Median IL-6 levels increased from day 0 to day 5 in both PRP-PC and BC-PC. In PRP-PC, IL-8 increased from <3 pg/ml on day 0 to 817 pg/ml on day 5, while in BC-PC the corresponding levels were 10 and 346.5 pg/ml, respectively. No significant increase in levels of TNF-alpha was observed in BC-PC during storage period, while levels increased significantly in PRP-PC on day 1 only. There was no significant change in the levels of all three cytokines in leucofiltered PCs over 5 days of storage. INTERPRETATION &CONCLUSION:
Findings of our study showed that method of preparation and WBC content are the critical factors in determining the cytokine levels in stored PCs.
Full text:
1
Index:
IMSEAR
Main subject:
Time Factors
/
Blood Platelets
/
Blood Preservation
/
Humans
/
Cell Separation
/
Prospective Studies
/
Cytokines
/
Interleukin-8
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
Type of study:
Observational_studies
Language:
En
Year:
2006
Type:
Article