Rotavirus-specific Salivary and Fecal IgA in Indian Children and Adults.
Indian Pediatr
; 2016 Jul; 53(7): 601-606
Article
in En
| IMSEAR
| ID: sea-179122
ABSTRACT
Objective:
To compare serum, salivary and fecal IgA responses in infants and adults following rotavirus vaccination. Studydesign:
Laboratory testing of samples from clinical trials.Setting:
Medical College Hospital.Participants:
13 healthy adult volunteers not given vaccine, 20 healthy adult volunteers given one dose of bovine rotavirus tetravalent vaccine (Shantha Biotechnics), and 88 infants given 3 or 5 doses of Rotarix. Outcomemeasures:
Serum, salivary and fecal IgA at one or more time points.Methods:
IgA antibodies were estimated in serum, saliva and fecal samples by enzyme-linked immunosorbent assay, and normalized to total IgA in saliva.Results:
In naturally infected adult volunteers, comparing serum and salivary IgA showed significant positive correlation (r=0.759; P=0.003). Of 20 vaccinated adults, complete samples showing change were available for 10; among them there was a significant positive correlation (P<0.05) between pre-vaccination serum and pre-vaccination salivary IgA but not between post-vaccination serum and post-vaccination salivary IgA. Of 88 infants given 3 or 5 doses of vaccine, 13 had more than 4-fold IgA response in serum, saliva and fecal samples, 6 had a 2-4 fold increases in all specimens. There was weak correlation between seroconversion rates measured by serum and salivary antibody responses. Salivary and stool assays were able to detect seroconversion in a few children in whom there was no detectable response in serum.Conclusions:
Evaluation of multiple samples is useful for intensive experimental study designs and may help improve our understanding of the induction and dynamics of immune responses to rotavirus vaccination.
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Index:
IMSEAR
Language:
En
Journal:
Indian Pediatr
Year:
2016
Type:
Article