Redox state of pyridine nucleotides, but not glutathione, regulate Ca2+ release by H2O2 from mitochondria of pulmonary smooth muscle.
Indian J Biochem Biophys
;
1996 Jun; 33(3): 218-22
Article
in English
| IMSEAR
| ID: sea-27312
ABSTRACT
Treatment of bovine pulmonary artery smooth muscle mitochondria with H2O2 stimulated oxidation of GSH and NAD(P)H along with an increase in Ca2+ release. Addition of oxaloacetate to mitochondrial suspension stimulated Ca2+ release and oxidation of NAD(P)H while GSH level remained unchanged. Subsequently, addition of beta-hydroxybutyrate which reduced mitochondrial pyridine nucleotides caused reuptake of the released Ca2+ without causing appreciable alteration of GSH level. Treatment of the mitochondria with 1,3-bis(2-dichloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase, significantly decreased GSH level without producing discernible change in Ca2+ release and NAD(P)H oxidation.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oxaloacetates
/
Oxidation-Reduction
/
Pulmonary Artery
/
Cattle
/
Kinetics
/
Carmustine
/
Calcium
/
Electron Transport Complex IV
/
Glutathione
/
Hydrogen Peroxide
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1996
Type:
Article
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