Improved presentation of antigenic sites in enzyme-linked immunosorbent assay: antigenicity of reduced and carboxymethylated riboflavin carrier protein.
Indian J Biochem Biophys
;
1993 Aug; 30(4): 209-13
Article
in English
| IMSEAR
| ID: sea-27667
ABSTRACT
Disulphide reduced and carboxymethylated riboflavin carrier protein (RCM-RCP), an unfolded derivative of chicken RCP, does not bind riboflavin and there is a drastic reduction in its ability to interact with antiserum to cRCP. Antibodies to RCM-RCP are directed against sequential epitopes(s). On radioiodination of RCM-RCP, a maximum of 30-50% binding at dilution of 1500 was obtained with rabbit antibodies RCM-RCP [n = 5]. However, high titer antibodies was obtained when radioiodinated RCM-RCP was immobilized on ELISA microtiter plates suggesting that immobilization of RCM-RCP leads to either preservation of antigenic sites or improved presentation of the antigenic determinants. An avidin-biotin system was utilized to develop an ELISA.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oxidation-Reduction
/
Membrane Transport Proteins
/
Riboflavin
/
Female
/
Enzyme-Linked Immunosorbent Assay
/
Carrier Proteins
/
Chickens
/
Immune Sera
/
Animals
/
Methylation
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1993
Type:
Article
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