Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 249-53
Article
in English
| IMSEAR
| ID: sea-28924
ABSTRACT
A recombinant vector for overproduction of the E. coli single stranded DNA binding protein (E. coli SSBP) has been constructed. An E. coli strain carrying this plasmid produces up to 150 mg pure SSBP per litre of bacterial culture in a laboratory shake flask. Electron microscopy of the single stranded DNA complexed with SSBP shows characteristic "beaded string"-like appearance. Strong clustering of protein molecules on ssDNA is indicative of a highly cooperative binding.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Promoter Regions, Genetic
/
Bacteriophage T7
/
DNA-Binding Proteins
/
Escherichia coli
/
Genetic Vectors
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1994
Type:
Article
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