Evaluation of polymerase chain reaction and restriction enzyme analysis for routine identification of mycobacteria: accuracy, rapidity, and cost analysis.
Southeast Asian J Trop Med Public Health
; 2005 Sep; 36(5): 1252-60
Article
in En
| IMSEAR
| ID: sea-30534
ABSTRACT
Polymerase chain reaction and restriction enzyme analysis (PCR-REA) of the hsp65 gene was evaluated for use as a routine identification method for identifying mycobacteria. The accuracy, rapidity, and cost were assessed compared with the conventional biochemical method. Five hundred and forty-one mycobacterial clinical isolates obtained from the Department of Microbiology, Faculty of Medicine at Siriraj Hospital, Mahidol University, were submitted for PCR-REA and biochemical identification. PCR-REA showed high concordant result with 100, 96.2, and 94.1% for identification of Mycobacterium tuberculosis, rapid- and slow-growing mycobacteria, respectively. Discordant results were obtained from 24 (4.4%) out of 541 isolates, consisting of 9 rapid growers (6 M. chelonae, 2 M. abscessus, and 1 M. fortuitum) and 15 slow growers (9 M. scrofulaceum, 2 M. gordonae, 1 M. avium, 1 M. kansasii, 1 M. malmoense, and 1 M. terrae complex). PCR-REA demonstrated not only accurate results but was also less expensive (2.1 US dollars/sample). This method was rapid with a turn-around time of 30 hours compared with 2-4 weeks for the conventional method.
Full text:
1
Index:
IMSEAR
Main subject:
Thailand
/
Bacterial Proteins
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Humans
/
DNA, Bacterial
/
Base Sequence
/
Restriction Mapping
/
Polymerase Chain Reaction
/
Reproducibility of Results
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DNA Primers
/
Chaperonins
Type of study:
Evaluation_studies
/
Health_economic_evaluation
/
Prognostic_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Southeast Asian J Trop Med Public Health
Year:
2005
Type:
Article