Surface and total tissue factor activity of endothelial cells.
Southeast Asian J Trop Med Public Health
; 1997 ; 28 Suppl 3(): 164-6
Article
in En
| IMSEAR
| ID: sea-31338
ABSTRACT
With a technic that was developed by us, we found that normal human umbilical vein endothelial cells (HUVEC) in culture characteristically had very little tissue factor (TF) activity either on the surface or in the cells which had been disrupted. In the presence of endotoxin (E. coli O26B6), a trigger for thrombosis in septicemic patients, we could not detect an increased TF activity of HUVEC on its surface. However, an increase in TF (total TF) was detected after disruption of the cells. The increase in total TF was dose-dependent. Endotoxin at the concentration of 10 micrograms/ml caused around 5 fold increase in total TF activity compared to that of HUVEC in the absence of endotoxin.
Full text:
1
Index:
IMSEAR
Main subject:
Humans
/
Thromboplastin
/
Endothelium, Vascular
/
Cells, Cultured
/
Endotoxins
Language:
En
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article