Schistosoma japonicum strains: differentiation by RAPD and SSR-PCR.
Southeast Asian J Trop Med Public Health
; 2002 Dec; 33(4): 720-4
Article
in En
| IMSEAR
| ID: sea-32027
ABSTRACT
Eight geographical isolates of Schistosoma japonicum from Taiwan and mainland China and one isolate of Schistosoma mansoni were studied by RAPD analysis using six arbitrary primers and SSR-PCR analysis using a (CA)8RY primer. The genetic distance was determined by the percentage of unshared bands. The RAPD and SSR-PCR results showed that the genetic distance between S. mansoni and S. japonicum was more than 0.900 and 0.850 respectively; the genetic distance between the eight geographical isolates of S. japonicum was 0.000 to 0.232 and 0.066 to 0.368 respectively. These results demonstrated the usefulness of RAPD and SSR-PCR for showing the differences of inter- and intra-species of Schistosoma. The results also suggest that there is genetic diversity among the different geographical strains of S. japonicum in China.
Full text:
1
Index:
IMSEAR
Main subject:
Phylogeny
/
Polymorphism, Genetic
/
Schistosoma japonicum
/
Species Specificity
/
Taiwan
/
Genetic Variation
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Schistosomiasis japonica
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
En
Journal:
Southeast Asian J Trop Med Public Health
Year:
2002
Type:
Article