The use of polymerase chain reaction (PCR) as a diagnostic tool for dengue virus.
Southeast Asian J Trop Med Public Health
; 1995 Dec; 26(4): 669-72
Article
in En
| IMSEAR
| ID: sea-32364
ABSTRACT
This study describes the use of polymerase chain reaction as a diagnostic tool for detecting and typing of dengue virus. PCR was compared against virus isolation. First RT-PCR was done using dengue consensus primers after which positive samples were subjected to RT-PCR using type-specific primers. This study shows that the local strains of the dengue virus could be detected using the chosen primers. Furthermore, RT-PCR was found to be more sensitive than virus isolation in identifying the dengue positive samples.
Full text:
1
Index:
IMSEAR
Main subject:
Humans
/
RNA, Viral
/
Serotyping
/
Case-Control Studies
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Random Amplified Polymorphic DNA Technique
/
Dengue Virus
/
Malaysia
Type of study:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Southeast Asian J Trop Med Public Health
Year:
1995
Type:
Article