Detection and structural analysis of abnormal hemoglobins found in Thailand.
Southeast Asian J Trop Med Public Health
; 1999 ; 30 Suppl 2(): 88-93
Article
in En
| IMSEAR
| ID: sea-35572
ABSTRACT
In Thailand, some 20 different abnormal hemoglobins have been found in the last 30 years. Most are rare except for Hb E and Hb Constant Spring, found with frequencies of 10-53% and 1-8% respectively in different parts of the country. Most mutations are point mutations, but C-terminal elongations and crossing-over are also found. Most mutations do not cause clinical problems, but some can give rise to mild thalassemia syndromes, or cause problems in association with thalassemia. Abnormal hemoglobins may often be diagnosed by electrophoresis, but some variants have the same mobility, so that other techniques are required, such as HPLC and the use of allele-specific polymerase reaction or oligonucleotide probes. Novel variants, not previously described in Thailand, require structural analysis at the protein and DNA level.
Full text:
1
Index:
IMSEAR
Main subject:
Thailand
/
Thalassemia
/
Humans
/
Hemoglobins, Abnormal
/
Polymerase Chain Reaction
/
Chromatography, High Pressure Liquid
/
Electrophoresis
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Mutation
Type of study:
Diagnostic_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Southeast Asian J Trop Med Public Health
Year:
1999
Type:
Article