Monoclonal antibodies against protein antigens of salmonellae causing paratyphoid fever and their diagnostic application.
Asian Pac J Allergy Immunol
; 1995 Jun; 13(1): 63-70
Article
in En
| IMSEAR
| ID: sea-36667
ABSTRACT
Hybrid clones producing monoclonal antibodies (MAbs) specific for Salmonella paratyphi A (72 clones), S. paratyphi B (9 clones) and S. paratyphi C (8 clones) were produced by using the affinity purified Salmonella protein (Bp) as immunogens. MAbs to S. paratyphi A and S. paratyphi B reacted specifically with the 52 kDa homologous flagellin protein components while those to S. paratyphi C reacted with a 61 kDa flagellin protein component. The MAbs against S. paratyphi A and S. paratyphi B were used to establish a double antibody sandwich ELISA for detection of the 52 kDa flagellin antigens in serum samples from patients with acute paratyphoid A and paratyphoid B fever. With this assay system, 6.25 ng per ml of flagellin antigens of S. paratyphi A and S. paratyphi B could be detected. However, the assay system could not detect the flagellin antigens in patients' sera. The presence of IgM antibodies to the 52 kDa antigens of S. paratyphi A and S. paratyphi B in the acute sera from paratyphoid A or paratyphoid B patients suggested that the 52 kDa protein components of both salmonellae are good immunogens for human and might be used as antigens for early diagnosis of paratyphoid A and paratyphoid B fever.
Full text:
1
Index:
IMSEAR
Main subject:
Salmonella
/
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Paratyphoid Fever
/
Flagellin
/
Antibodies, Monoclonal
/
Antigens, Bacterial
Type of study:
Screening_studies
Language:
En
Journal:
Asian Pac J Allergy Immunol
Year:
1995
Type:
Article