Identification of tumor markers for cholangiocarcinoma and evaluation of their diagnostic potential.
Asian Pac J Allergy Immunol
; 1994 Jun; 12(1): 73-81
Article
in En
| IMSEAR
| ID: sea-36904
ABSTRACT
Results obtained from studies using experimental animal model clearly showed that (1) A marker(s) for CCA does exist; 2) This marker is a glycoprotein with a molecular weight of 200 kDa; (3) It is produced and secreted in vitro by tumor cell lines; (4) It is highly immunogenic in mice and the MAb specific for this antigen is directed against the carbohydrate moiety; (5) This tumor antigen can be detected in serum and bile of tumor-bearing animals by a sandwich ELISA employing this MAb; (6) Kinetic studies show a gradual elevation of this antigen during tumor development; and (7) The elevation of this antigen can be detected at a time when no pathological changes have yet taken place, as judged by microscopic examination. Preliminary work from the human counterpart using human cholangiocarcinoma cell line showed promising results. CCA-specific antigen could be similarly identified and the MAbs produced were highly specific for this 160 kDa antigen.
Full text:
1
Index:
IMSEAR
Main subject:
Rabbits
/
Bile Duct Neoplasms
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Bile Ducts, Intrahepatic
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Humans
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Tumor Cells, Cultured
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Glycoproteins
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Biomarkers, Tumor
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Cricetinae
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Mesocricetus
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Cholangiocarcinoma
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Asian Pac J Allergy Immunol
Year:
1994
Type:
Article