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Curing of symbiotic plasmid of Mesorhizobium huakuii subsp. rengei isolated from Astragalus sinicus.
Indian J Exp Biol ; 2003 Aug; 41(8): 912-4
Article in En | IMSEAR | ID: sea-56474
ABSTRACT
Astragalus sinicus (Chinese Milk vetch), a green manure leguminous plant, harbors Mesorhizobium huakuii subsp. rengei strain B3 in the root nodules. The visualization of symbiotic plasmid of strain B3 showed the presence of one sym plasmid of about 425 kbp. Curing of sym plasmid by temperature and acrydine orange was studied. Growing rhizobial cells at high temperature (37 degrees C) or treating the cells with acrydine orange at 50 mg/l eliminated sym plasmid of M. huakuii strain B3, which was confirmed by sym plasmid visualization and plant infection test of cured strains.
Subject(s)
Full text: 1 Index: IMSEAR Main subject: Plasmids / Rhizobium / Symbiosis / Plant Roots / Astragalus Plant / Nitrogen Fixation Language: En Journal: Indian J Exp Biol Year: 2003 Type: Article
Full text: 1 Index: IMSEAR Main subject: Plasmids / Rhizobium / Symbiosis / Plant Roots / Astragalus Plant / Nitrogen Fixation Language: En Journal: Indian J Exp Biol Year: 2003 Type: Article