Experimental amebiasis: molecular weight analysis of Entamoeba histolytica antigens recognized by IgG antibodies.
Indian J Exp Biol
;
1999 Aug; 37(8): 803-6
Article
in English
| IMSEAR
| ID: sea-56929
ABSTRACT
The reactivity of sera from experimentally infected animal was studied from 5-60 days postinoculation to determine which of the E. histolytica antigens are recognized frequently to infection. Crude extract of E. histolytica trophozoites was used and sera were examined by immunoelectrotransference assay. It was observed that sera recognized polypeptide with 70 kDa molecular mass after 15 days postinoculation onward and later 14 to 24 polypeptide with molecular weight of 110-22 kDa were recognized. All the amebic antigens (polypeptides) could be recognized by sera till 60 days postinoculated animals. Significance of expression of different amebic polypeptides in terms of pathogenesis needs further investigations.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Immunoglobulin G
/
Entamoeba histolytica
/
Entamoebiasis
/
Enteritis
/
Animals
/
Mice
/
Mice, Inbred C3H
/
Molecular Weight
/
Antigen-Antibody Reactions
/
Antigens, Protozoan
Language:
English
Journal:
Indian J Exp Biol
Year:
1999
Type:
Article
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