Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1.
Indian J Exp Biol
;
2005 Sep; 43(9): 838-40
Article
in English
| IMSEAR
| ID: sea-58585
ABSTRACT
Brucella melitensis is an organism of paramount zoonotic importance. The 28 kDa outer membrane protein (OMP) is one of the immunodominant antigens of B. melitensis. The gene encoding 28 kDa OMP (omp28) has been amplified from B. melitensis Rev. 1 strain. A PCR product of 753 bp, encoding complete omp28 gene of B. melitensis, was obtained. The gene was further cloned and sequenced. The nucleotide sequence of B. melitensis Rev. 1 strain showed substitution of 2 nucleotides from that of 16M strain.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmids
/
Bacterial Outer Membrane Proteins
/
Recombinant Proteins
/
Molecular Sequence Data
/
Brucella Vaccine
/
Base Sequence
/
Cell Membrane
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Protein Structure, Tertiary
Language:
English
Journal:
Indian J Exp Biol
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS