Potentiality of a new compound for in vitro differentiation between halophilic and non-halophilic vibrios.
Indian J Exp Biol
; 2002 Feb; 40(2): 220-2
Article
in En
| IMSEAR
| ID: sea-59323
ABSTRACT
Sensitivity of 21 halophilic vibrios and 16 clinical isolates of non-halophilic vibrios was determined against a new possible antivibrio agent, a pyrimidine analogue, 4, 6-dimethylpyrimidine -2-thiol (4,6-DMPT). It appeared to be a vibriocidal agent, having a mean MIC and MBC of 32 microg/ml for halophilic strains and 64 microg/ml for non-halophilic strains and an LD50 of 300 mg/Kg body weight of mice. Thus, 4,6-DMPT may help an in vitro distinction between halophilic and non-halophilic vibrios. Sensitivity of these strains was also studied with respect to pteridine, crystal violet and Tween 80 hydrolysis as further markers distinguishing between these 2 groups which could also be differentiated by their growth on TCBS or/and CLED media.
Full text:
1
Index:
IMSEAR
Main subject:
Polysorbates
/
Pteridines
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Pyrimidines
/
Surface-Active Agents
/
Vibrio cholerae
/
Vibrio parahaemolyticus
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Microbial Sensitivity Tests
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Sensitivity and Specificity
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Gentian Violet
/
Hydrolysis
Type of study:
Diagnostic_studies
Language:
En
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article