Studies on diagnostic potential of secretory antigens of Mycobacterium bovis.
Indian J Exp Biol
; 2006 Jan; 44(1): 14-8
Article
in En
| IMSEAR
| ID: sea-62003
ABSTRACT
Culture filtrate proteins (CFPs) of M. bovis were produced by culturing the organism for a period of 3 weeks. These CFPs elicited a good delayed hypersensitivity (DTH) reaction in cattle and guinea pig. However, CFPs showed least cross-reactivity when tested in guinea pigs sensitized with M. bovis BCG and M. phlei. CFPs were found to be well recognized by T-cells of single intradermal test (SIDT) positive cattle and were also sero-reactive. Lymphocyte transformation assay revealed the culture filtrate antigens as major T-cell antigens. CFPs induced a good interferon gamma release as compared to conventional antigen, PPD, thereby highlighting their diagnostic capabilities.
Full text:
1
Index:
IMSEAR
Main subject:
Tuberculosis, Bovine
/
Cattle
/
Lymphocyte Activation
/
Interferon-gamma
/
Guinea Pigs
/
Hypersensitivity, Delayed
/
Animals
/
Antibodies, Bacterial
/
Mycobacterium bovis
/
Antigens, Bacterial
Language:
En
Journal:
Indian J Exp Biol
Year:
2006
Type:
Article