Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm.
Indian J Exp Biol
; 2004 Jun; 42(6): 567-9
Article
in En
| IMSEAR
| ID: sea-63185
ABSTRACT
Biliary, ureteric and intestinal colic are extremely common clinical conditions associated with smooth muscle spasm. In the present study, antispasmodic activity was carried out against acetylcholine (10-640 ng/ml)-induced contractions on guinea pig ileum. Acetylcholine (10-640 ng/ml) induced concentration-dependent contraction of smooth muscle. Diclofenac, in varying concentration (9.4 x 10(-5) mol/l and 14.1 x 10(-5) mol/l) shifted the concentration response curve of acetylcholine to the right without suppressing the maximal response. However, in higher concentration diclofenac (18.9 x 10(-5) mol/l) blocked the response in an unsurmountable fashion. Further, analgin (11.09 x 10(-5), 16.63 x 10(-5) and 22.18 x 10(-5) mol/l) in equimolar concentrations did not alter the concentration response curve of acetylcholine, but in higher concentration analgin (44.36 x 10(-5) mol/l) also blocked the response in an unsurmountable fashion. Pitofenone (2.5 x 10(-6) mol/l) also, shifted the concentration response curve of acetylcholine to right in a parallel fashion with no change in maximal response. The present study confirms the potent antispasmodic activity of diclofenac-pitofenone combination in comparison to analgin-pitofenone in molar equivalent concentration (in comparison to diclofenac) against acetylcholine-induced contractions of guinea pig ileum.
Full text:
1
Index:
IMSEAR
Main subject:
Parasympatholytics
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Spasm
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Benzophenones
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Female
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Male
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Acetylcholine
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Anti-Inflammatory Agents, Non-Steroidal
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Diclofenac
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Dipyrone
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Cholinergic Agents
Language:
En
Journal:
Indian J Exp Biol
Year:
2004
Type:
Article