Single chain antibody fragment display systems: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 3681-3694, 2023.
Article
in Zh
| WPRIM
| ID: wpr-1007985
Responsible library:
WPRO
ABSTRACT
Single chain antibody fragment (scFv) is a small molecule composed of a variable region of heavy chain (VH) and a variable region of light chain (VL) of an antibody, and these two chains are connected by a flexible short peptide. scFv is the smallest functional fragment with complete antigen-binding activity, which contains both the antibody-recognizing site and the antigen-binding site. Compared with other antibodies, scFv has the advantages of small molecular weight, strong penetration, low immunogenicity, and easy expression. Currently, the most commonly used display systems for scFv mainly include the phage display system, ribosome display system, mRNA display system, yeast cell surface display system and mammalian cell display system. In recent years, with the development of scFv in the field of medicine, biology, and food safety, they have also attracted much attention in the sectors of biosynthesis and applied research. This review summarizes the advances of scFv display systems in recent years in order to facilitate scFv screening and application.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Immunoglobulin Variable Region
/
Immunoglobulin Fragments
/
Peptide Library
/
Single-Chain Antibodies
/
Mammals
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2023
Type:
Article