Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 155-165, 2020.
Article
in En
| WPRIM
| ID: wpr-1010522
Responsible library:
WPRO
ABSTRACT
Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to analyze the protein expression levels and localization of P2X7R. STZ-induced mice expressed increased P2X7R in the dorsal horn of the lumbar spinal cord during MA. Mice injected intrathecally with a selective antagonist of P2X7R and P2X7R knockout (KO) mice both presented attenuated progression of MA. Double-immunofluorescent labeling demonstrated that P2X7R-positive cells were mostly co-expressed with Iba1 (a microglia marker). Our results suggest that P2X7R plays an important role in the development of MA and could be used as a cellular target for treating PDN.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Quinolines
/
Spinal Cord
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Streptozocin
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Diabetes Mellitus, Experimental
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Diabetes Mellitus, Type 1
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Diabetic Neuropathies
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Receptors, Purinergic P2X7
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Hyperalgesia
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Acetamides
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Mice, Inbred C57BL
Limits:
Animals
Language:
En
Journal:
Journal of Zhejiang University. Science. B
Year:
2020
Type:
Article