Different Expression of Extracellular Matrix Genes: Primary vs. Recurrent Disc Herniation
Journal of Korean Neurosurgical Society
; : 26-29, 2010.
Article
in En
| WPRIM
| ID: wpr-101198
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: Recurrent lumbar disc herniation has been reported to occur in 5% to 15% of surgically treated primary lumbar disc herniation cases. We investigated the molecular biologic characteristics of primary herniated discs and recurrent discs to see whether the recurrent discs has the similar biological features with primary herniated discs. METHODS: Primary herniated disc and recurrent disc cells were obtained by discectomy of lumbar disc patients and cells were isolated and then taken through monolayer cultures. We compared chondrogenic and osteogenic mRNA gene expression, and western blot between the two groups. RESULTS: The mRNA gene expression of recurrent disc cells were increased 1.47* times for aggrecan, 1.38 times for type I collagen, 2.04 times for type II collagen, 1.22 times for both Sox-9 and osteocalcin, and 1.31 times for alkaline phosphatase, respectively, compared with the primary herniated lumbar disc cells (*indicates p < 0.05). Western blot results for each aggrecan, type I collagen, type II collagen, Sox-9, osteocalcin, and alkaline phosphatase were similar between the primary herniated disc cells and recurrent disc cells. CONCLUSION: These results indicate that the recurrent disc cells have similar chondrogenic and osteogenic gene expression compared to primary herniated disc cells. Therefore, we assumed that the regeneration of remaining discs could fill the previous discectomy space and also it could be one of the factors for disc recurrence especially in the molecular biologic field.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Recurrence
/
Regeneration
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Population Characteristics
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RNA, Messenger
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Gene Expression
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Osteocalcin
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Blotting, Western
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Collagen
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Durapatite
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Diskectomy
Limits:
Humans
Language:
En
Journal:
Journal of Korean Neurosurgical Society
Year:
2010
Type:
Article