Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis
Experimental & Molecular Medicine
; : 427-435, 2011.
Article
in En
| WPRIM
| ID: wpr-102680
Responsible library:
WPRO
ABSTRACT
Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatographymass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with < 1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. alpha2-HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and alpha-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P < 0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pre-Eclampsia
/
Alpha-Globulins
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Molecular Sequence Data
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Blood Proteins
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Biomarkers
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Case-Control Studies
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Amino Acid Sequence
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Insulin-Like Growth Factor Binding Proteins
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Proteome
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Retinol-Binding Proteins, Plasma
Type of study:
Observational_studies
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Prognostic_studies
Limits:
Adult
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Female
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Humans
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Pregnancy
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2011
Type:
Article