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The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines
Experimental & Molecular Medicine ; : 401-410, 2011.
Article in English | WPRIM | ID: wpr-102683
ABSTRACT
Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Azacitidine / Viral Proteins / RNA, Viral / B-Lymphocytes / DNA Modification Methylases / Gene Expression Regulation, Viral / Cell Line / Promoter Regions, Genetic / Herpesvirus 4, Human / DNA Methylation Limits: Humans Language: English Journal: Experimental & Molecular Medicine Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Azacitidine / Viral Proteins / RNA, Viral / B-Lymphocytes / DNA Modification Methylases / Gene Expression Regulation, Viral / Cell Line / Promoter Regions, Genetic / Herpesvirus 4, Human / DNA Methylation Limits: Humans Language: English Journal: Experimental & Molecular Medicine Year: 2011 Type: Article