The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines
Experimental & Molecular Medicine
; : 401-410, 2011.
Article
in En
| WPRIM
| ID: wpr-102683
Responsible library:
WPRO
ABSTRACT
Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Azacitidine
/
Viral Proteins
/
RNA, Viral
/
B-Lymphocytes
/
DNA Modification Methylases
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Gene Expression Regulation, Viral
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Cell Line
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Promoter Regions, Genetic
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Herpesvirus 4, Human
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DNA Methylation
Limits:
Humans
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2011
Type:
Article