Detection of mosquito-borne viruses based on high-throughput sequencing / 中华检验医学杂志

ABSTRACT

Mosquitoes are widely distributed around the world and carry a diverse array of viruses. Among these, mosquito-borne viruses possess the capability to replicate in both blood-sucking arthropods and vertebrate cells, rendering them significant threats to public health. For many years, the surveillance of mosquito-borne viruses mainly relied on traditional methods such as virus isolation and culture. The emergence of high-throughput sequencing (HTS) technology has not only revealed the entire spectrum of mosquito virome, but also provided a basic methodology for the rapid screening of mosquito-borne pathogens and identification of new species. Consequently, HTS has facilitated the prevention and control of mosquito-borne diseases. This review aims to describe the methods and application of HTS in the detection of mosquito-borne viruses, while also summarizing the advantages and disadvantages of HTS-based detection of mosquito-borne pathogens compared to traditional methods.