Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
The Korean Journal of Parasitology
; : 237-241, 2013.
Article
in En
| WPRIM
| ID: wpr-103947
Responsible library:
WPRO
ABSTRACT
Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from 10(-1) to 10(-5) ng/microl for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of 63degrees C by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha (EF1alpha) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Temperature
/
Time Factors
/
Veterinary Medicine
/
Molecular Sequence Data
/
Sensitivity and Specificity
/
Giardiasis
/
Giardia lamblia
/
Sequence Analysis, DNA
/
Nucleic Acid Amplification Techniques
/
Molecular Diagnostic Techniques
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2013
Type:
Article