Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria / 中山大学学报(医学科学版)

ABSTRACT

ObjectiveTo investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells， as well as the antibacterial efficacy of baicalein against prevalent oral bacteria， and to elucidate the underlying mechanisms. MethodsMC3T3-E1 cells were exposed to different concentrations of baicalein （0， 6， 12， 18， and 24 μmol/L） and cell viability was determined by using the CCK-8 assay. Alkaline phosphatase （ALP） activity of MC3T3-E1 cells following osteogenic induction was assessed. RT-PCR was used to examine the expression of RunX2， BMP2， and Osterix. After 24 hours of treatment， the antibacterial potential of baicalein against Escherichia coli， Staphylococcus Aureus and Streptococcus Sanguis was evaluated by using the K-B paper disk method. ResultsBaicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation. Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells， upregulated BMP2 and Osterix expression， downregulated RunX2 expression， significantly inhibited the proliferation of Staphylococcus Aureus and Streptococcus Sanguis （P < 0.05）. ConclusionsBaicalein at an optimal concentration （18 μmol/L） demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bacteria， including Staphylococcus Aureus and Streptococcus Sanguis.