Cloning of canine GM-CSF and SCF genes
Journal of Veterinary Science
; : 159-166, 2001.
Article
in En
| WPRIM
| ID: wpr-109439
Responsible library:
WPRO
ABSTRACT
Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation of granulocytes and macrophages and regulates the biological functions expressed by mature cells of these lineages. Stem cell factor (SCF) is a multifunctional cytokine involved in hematopoiesis, melanogenesis and gametogenesis. In order to determine the complementary DNA (cDNA) of canine GM-CSF and canine SCF, cDNA clones were generated from lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMCs) and bone marrow cells by reverse transcription PCR amplification. The canine GM-CSF cDNA obtained in this study contains an open reading frame encoding 144 amino acid residues and has 53-75% homology with those of human, cat, sheep, pig, cow and mouse, Canine SCF cDNA consist of an open reading frame encoding 274 amino acid residues and shares 81-92% homology with those of human, cat, pig, cow and mouse.
Full text:
1
Index:
WPRIM
Main subject:
Swine
/
Codon
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Sheep
/
Molecular Sequence Data
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Base Sequence
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Gene Amplification
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Granulocyte-Macrophage Colony-Stimulating Factor
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Open Reading Frames
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Sequence Alignment
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Amino Acid Sequence
Limits:
Animals
/
Humans
Language:
En
Journal:
Journal of Veterinary Science
Year:
2001
Type:
Article