Detecting Bacterial Growth in Continuous Ambulatory Peritoneal Dialysis Effluent Using Two Culture Methods
The Korean Journal of Internal Medicine
;
: 82-85, 2010.
Article
in English
| WPRIM
| ID: wpr-10972
ABSTRACT
BACKGROUND/AIMS:
The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD).METHODS:
One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cases, 5 to 10 mL of dialysate was inoculated into a pair of BacT/Alert blood culture bottles, and 50 mL of centrifuged dialysate was simultaneously inoculated into a solid culture media for conventional culture. The results were compared to those of the conventional culture method. Isolated microorganisms were compared between the two methods.RESULTS:
The blood culture method was positive in 78.6% (88 / 112) of dialysate specimens and the conventional culture method in 50% (56 / 112, p < 0.001).CONCLUSIONS:
The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peritonitis
/
Dialysis Solutions
/
Microbiological Techniques
/
Sensitivity and Specificity
/
Peritoneal Dialysis, Continuous Ambulatory
/
Gram-Positive Bacterial Infections
/
Gram-Negative Bacterial Infections
/
Culture Media
/
Kidney Failure, Chronic
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
The Korean Journal of Internal Medicine
Year:
2010
Type:
Article
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