Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720
The Korean Journal of Parasitology
; : 637-644, 2013.
Article
in En
| WPRIM
| ID: wpr-118762
Responsible library:
WPRO
ABSTRACT
This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oligodeoxyribonucleotides
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Immunoglobulin G
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Immunoglobulin M
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Antibodies, Helminth
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Oleic Acids
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Adjuvants, Immunologic
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Th2 Cells
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Th1 Cells
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Gnathostoma
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Mannitol
Limits:
Animals
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2013
Type:
Article