Changes in the Physical Properties of Irradiated Red Blood Cells / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 129-137, 2007.
Article
in Ko
| WPRIM
| ID: wpr-118889
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Irradiation of cellular blood products is the current method used for the prevention of transfusion-associated graft-versus-host disease. However, irradiation has been shown to cause biochemical changes in stored red blood cells (RBCs) and to generate reactive oxygen species (ROS). Irradiation-induced biochemical changes and oxidation damage of the RBCs is closely related to the deformability of these cells. Furthermore, deformation of the RBCs may lead to alterations in the post-transfusion viability of stored RBCs. In this study, we evaluated the physical properties of irradiated RBCs.METHODS:
Citrate phosphate dextrose adenine-1 preserved RBC concentrates were irradiated with a minimum dose of 25 Gy and stored at 4oC for 28 days. The deformation of the RBCs was measured by a microfluidic ektacytometer (RheoScan-D). To examine the effect of oxidative stress, the RBC concentrates were exposed to oxidative stress using FeSO4 and ascorbic acid.RESULTS:
The deformability threshold of the irradiated RBCs was significantly lower than that of the control RBCs on day 21 of storage and later (P<0.05). The deformability threshold of the RBCs exposed to oxidative stress was lower than that of control RBCs, and the difference was significant on day 21 of storage and later (P<0.05). For up to 21 days of storage, the deformability of control and irradiated RBCs were maintained; however, they were significantly decreased at 28 days of storage compared with 1 day of storage (P<0.05).CONCLUSION:
Irradiation reduced the threshold of RBC deformability during storage. In accordance with the effect of irradiation, oxidative stress affected the RBC deformability. Therefore, a ROS scavenger may play a protective role against deformation of irradiated RBC concentrates. Further evaluation will be required for confirmation and clarification of these findings.Key words
Full text:
1
Index:
WPRIM
Main subject:
Ascorbic Acid
/
Reactive Oxygen Species
/
Oxidative Stress
/
Citric Acid
/
Microfluidics
/
Erythrocytes
/
Glucose
/
Graft vs Host Disease
Language:
Ko
Journal:
Korean Journal of Blood Transfusion
Year:
2007
Type:
Article