Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2)
Nutrition Research and Practice
; : 180-183, 2007.
Article
in En
| WPRIM
| ID: wpr-122437
Responsible library:
WPRO
ABSTRACT
Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At 80 microgram/mL of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of 200 microgram/mL of each polysaccharide isolate to the cell line containing 80 microgram/mL of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Polysaccharides
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Agaricus
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Water
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Cell Line
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Shiitake Mushrooms
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Cordyceps
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Agaricales
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Agrocybe
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Flammulina
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Liver
Limits:
Animals
Language:
En
Journal:
Nutrition Research and Practice
Year:
2007
Type:
Article