The upstream sequence of Mycobacterium leprae 18-kDa gene confers transcription repression activity in orientation-independent manner
Experimental & Molecular Medicine
; : 510-514, 2004.
Article
in En
| WPRIM
| ID: wpr-13641
Responsible library:
WPRO
ABSTRACT
In order to understand the role of the upstream region of the Mycobacterium leprae 18-kDa gene on the gene regulation, the region was divided into two at the -50 position from the first start codon of the gene and their effect on transcription was examined by using a LacZ transcriptional reporter gene assay. The presence of each of these two regions conferred transcription repression not only on its cognate M. lepraerae 18-kDa gene promoter, but also on a heterologous promoter such as the Mycobacterium bovis BCG hsp65 gene promoter. Moreover, it was found that these regions could confer transcription repression activity in both cases in an orientation-independent manner. Thus, these results indicate that the upstream region of the M. leprae 18-kDa gene harbors transcription repression responsive element(s) acting as an operator and can be further divided into two separately functional regions, suggesting a bipartite structure of the element(s). The identification of transcription repression activity of the upstream region in the M. leprae 18-kDa gene will contribute greatly for the understanding of the 18-kDa gene regulation mechanism, and provide also useful information for the manipulation of mycobacterium gene expression.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Bacterial Proteins
/
Transcription, Genetic
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Down-Regulation
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Gene Expression Regulation, Bacterial
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Response Elements
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Mycobacterium leprae
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2004
Type:
Article