Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats
Nutrition Research and Practice
; : 144-149, 2015.
Article
in En
| WPRIM
| ID: wpr-143314
Responsible library:
WPRO
ABSTRACT
BACKGROUND/OBJECTIVE: The aim of this study was to examine the effect of high dietary methionine (Met) consumption on plasma and hepatic oxidative stress and dyslipidemia in chronic ethanol fed rats. MATERIALS/METHODS: Male Wistar rats were fed control or ethanol-containing liquid diets supplemented without (E group) or with DL-Met at 0.6% (EM1 group) or 0.8% (EM2 group) for five weeks. Plasma aminothiols, lipids, malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase were measured. Hepatic folate, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured. RESULTS: DL-Met supplementation was found to increase plasma levels of homocysteine (Hcy), triglyceride (TG), total cholesterol (TC), and MDA compared to rats fed ethanol alone and decrease plasma ALT. However, DL-Met supplementation did not significantly change plasma levels of HDL-cholesterol, cysteine, cysteinylglycine, and glutathione. In addition, DL-Met supplementation increased hepatic levels of folate, SAM, SAH, and SAM:SAH ratio. Our data showed that DL-Met supplementation can increase plasma oxidative stress and atherogenic effects by elevating plasma Hcy, TG, and TC in ethanol-fed rats. CONCLUSION: The present results demonstrate that Met supplementation increases plasma oxidative stress and atherogenic effects by inducing dyslipidemia and hyperhomocysteinemia in ethanol-fed rats.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plasma
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Aspartate Aminotransferases
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S-Adenosylhomocysteine
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S-Adenosylmethionine
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Triglycerides
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Cholesterol
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Rats, Wistar
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Oxidative Stress
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Hyperhomocysteinemia
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Cysteine
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Nutrition Research and Practice
Year:
2015
Type:
Article