Effects of 5-hydroxytryptamine on Rocuronium-induced Neuromuscular Blockade in a Rat Phrenic Nerve-hemidiaphragm Preparation / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 438-442, 2007.
Article
in Korean
| WPRIM
| ID: wpr-161788
ABSTRACT
BACKGROUND:
The 5-hydroxytryptamine 3 receptor (5-HT3R) is a member of a superfamily of ligand-gated ion channels which has structural similarities and common evolutionary origin to those of the nicotinic acetylcholine receptor (nAChR). 5-hydroxytryptamine (5-HT) and muscle relaxants may have cross reaction. Rocuronium is a non-depolarizing neuromuscular blocking agent which has a rapid onset. The aim of this study was to examined the effects of 5-HT on rocuronium-induced neuromuscular blockade in a rat phrenic nerve-hemidiaphragm preparation.METHODS:
Institutional approval was obtained for the experimental procedure. Fifty male Sprague-Dawley rats (150-200 g) were divided into 5 groups; the control, and 0.1, 1, 10, and 20microgram/ml of 5-HT. The animals were injected with phentobarbital at 40 mg/kg into the peritoneal cavity. The hemidiaphragm with the phrenic nerve was dissected and then mounted in a bath containing 100 ml Krebs solution at room temperature. The phrenic nerve was stimulated at the supramaximal intensity using a Grass(R) S88 stimulator via an SIU5 isolation unit. The twitch height was measured and recorded using a precalibrated Grass(R) FT88 force displacement transducer and recorded with a Grass(R) 79 polygraph, respectively. In the cumulative dose-response study, the rocuronium 100microgram/dl and each dose of 5-HT were administered simultaneously administered, with additional 50microgram/dl incremental doses of rocuronium were added to obtain grteater than 95% neuromuscular twitch inhibition. The ED5, ED50, ED90, and ED95 of rocuronium in each group were calculated using a probit model.RESULTS:
The ED50, ED90, and ED95 of rocuronium were significantly reduced in 5-HT 20microgram/ml group (P < 0.05), but no differences were observed with the other 5-HT groups compared to the control groups.CONCLUSIONS:
5-HT at 20microgram/ml enhanced the neuromuscular blockade of rocuronium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peritoneal Cavity
/
Phrenic Nerve
/
Transducers
/
Baths
/
Serotonin
/
Receptors, Nicotinic
/
Rats, Sprague-Dawley
/
Neuromuscular Blockade
/
Cross Reactions
/
Ligand-Gated Ion Channels
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Anesthesiology
Year:
2007
Type:
Article
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