Emergence and Wide Dissemination of CTX-M-type ESBLs, and CMY-2- and DHA-1-type AmpC beta-Lactamases in Korean Respiratory Isolates of Klebsiella pneumoniae
Journal of Korean Medical Science
; : 961-965, 2005.
Article
in En
| WPRIM
| ID: wpr-16324
Responsible library:
WPRO
ABSTRACT
Respiratory isolates of Klebsiella pneumoniae in Korea during 2002-2003 were studied to determine the prevalence and types of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamases (PABLs). ESBL-production was tested by double-disk synergy, and genotypes of beta-lactamases were determined by PCR and sequencing. ESBLs were detected in 28.4% of 373 isolates, and the most prevalent types were SHV-12 (63 isolates) and CTX-M-14 (9 isolates). Forty of 75 ESBL-producers (53.5%) also had PABLs: 21 isolates with CMY-2-like, 17 with DHA-1-like. Pulsed-field gel electrophoresis showed 19 types and 25 of 74 isolates had an identical pattern, indicating nosocomial spread. Dissemination of ESBL- and PABL-producing K. pneumoniae strains in Korea is a particular concern, as it limits the choice of antimicrobial agents for treatment of infections.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Respiratory Tract Infections
/
Bacterial Proteins
/
Beta-Lactamases
/
Klebsiella Infections
/
DNA, Bacterial
/
Base Sequence
/
Cross Infection
/
Electrophoresis, Gel, Pulsed-Field
/
Drug Resistance, Bacterial
/
Genes, Bacterial
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2005
Type:
Article