Urinary Nucleic Acid TSPAN13-to-S100A9 Ratio as a Diagnostic Marker in Prostate Cancer
Journal of Korean Medical Science
; : 1784-1792, 2015.
Article
in En
| WPRIM
| ID: wpr-164157
Responsible library:
WPRO
ABSTRACT
The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed. Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0.037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0.001 and P = 0.013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.898 and 0.676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Prostate
/
Prostatic Hyperplasia
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Prostatic Neoplasms
/
RNA, Messenger
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RNA, Neoplasm
/
Nucleic Acids
/
Biomarkers, Tumor
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ROC Curve
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Cohort Studies
/
Oligonucleotide Array Sequence Analysis
Type of study:
Diagnostic_studies
/
Etiology_studies
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Incidence_studies
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Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Aged
/
Aged80
/
Humans
/
Male
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2015
Type:
Article