Regulation of type I collagen and interstitial collagenase mRNA expression in human dermal fibroblasts by colchicine and D-penicillamine
Yonsei Medical Journal
; : 490-495, 1999.
Article
in En
| WPRIM
| ID: wpr-164914
Responsible library:
WPRO
ABSTRACT
Sclerosis is a disease process in which idiopathic hardening occurs in the skin and/or internal organs as a result of the accumulation of type I collagen, induced mainly by transforming growth factor-beta. Colchicine and D-penicillamine are widely used for its treatment. Their effects are known to be due to post-translational down-regulation of type I collagen synthesis, with colchicine also up-regulating interstitial collagenase. To determine whether or not they have any pre-translational effect on type I collagen and MMP-1, and also to observe their effects on the action of TGF-beta, cultured neonatal foreskin fibroblasts were treated with colchicine and D-penicillamine, singly and together. The amount of type I collagen and MMP-1 mRNA were quantitated by Northern blot hybridization. Colchicine suppresses the basal level of type I collagen mRNA but minimally stimulates the mRNA expression of MMP-1, whereas D-penicillamine does not have any significant effects on either. Colchicine was also able to significantly suppress the TGF-beta-induced up-regulation of type I collagen mRNA expression.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Penicillamine
/
Skin
/
RNA, Messenger
/
Cells, Cultured
/
Colchicine
/
Gene Expression Regulation
/
Transforming Growth Factor beta
/
Collagen
/
Matrix Metalloproteinase 1
/
Fibroblasts
Limits:
Humans
Language:
En
Journal:
Yonsei Medical Journal
Year:
1999
Type:
Article