Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers
Journal of Lung Cancer
; : 111-113, 2006.
Article
in Ko
| WPRIM
| ID: wpr-167585
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. In addition to its role in oxidative phosphorylation, release of cytochrome c from the mitochondrial intermembrane space results in nuclear apoptosis. The aim of this study was to explore whether alteration of cytochrome c gene mutation is a characteristic of human non-small cell lung cancers (NSCLC). MATERIALS ANDMETHODS:
In the current study, to detect the somatic mutations in the DNA sequences encoding cytochrome c in 48 NSCLCs, we used polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP), and DNA sequencing.RESULTS:
The SSCP analysis revealed no mutation in the entire coding regions and all splice sites of human cytochrome c gene in the 48 NSCLCs.CONCLUSION:
The data presented here indicate that the pro-apoptotic cytochrome c may not be somatically mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of cytochrome c gene in the mechanisms for evading apoptosis.Key words
Full text:
1
Index:
WPRIM
Main subject:
Oxidative Phosphorylation
/
Base Sequence
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Apoptosis
/
Carcinoma, Non-Small-Cell Lung
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Polymorphism, Single-Stranded Conformational
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Cytochromes
/
Cytochromes c
/
Clinical Coding
Limits:
Humans
Language:
Ko
Journal:
Journal of Lung Cancer
Year:
2006
Type:
Article