Efficacy of Purified Vi Polysaccharide Typhoid Vaccine
Journal of Veterinary Science
; : 67-70, 2002.
Article
in En
| WPRIM
| ID: wpr-172834
Responsible library:
WPRO
ABSTRACT
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microgram / 0.5 ml / mouse. The ELISA titer (0.5097+/-0.0606) was 4 times in the group treated with high dose (0.25 microgram/0.5ml) as in control (0.1113+/-0.0110). Six major protein bands of 66, 55, 35, 33, 18, and 9 kd were detected in Western blot analysis with serum of a vaccine treated mouse, whereas only one weak band of about 35 kd was detected with serum of a control mouse. We concluded that typhoid vaccine produced by purifying Vi antigen of S. typhi very effectively prevent S. typhi infection in mice.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Polysaccharides, Bacterial
/
Salmonella typhi
/
Typhoid Fever
/
Enzyme-Linked Immunosorbent Assay
/
Typhoid-Paratyphoid Vaccines
/
Logistic Models
/
Blotting, Western
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Lethal Dose 50
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Mice, Inbred BALB C
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Antibodies, Bacterial
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2002
Type:
Article