Expression of Toll-like Receptor 2 in Cultured Human Keratinocytes: The Effect of Bacterial Antigens, Cytokines and Calcium Concentration
Annals of Dermatology
; : 337-344, 2009.
Article
in En
| WPRIM
| ID: wpr-174315
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Toll-like receptors (TLRs) are expressed by human epidermal keratinocytes and are involved in immune responses. OBJECTIVE: The goal of this was to investigate the expression of TLR2 in response to bacterial antigens, cytokines, and different calcium concentrations. METHODS: The expression of TLR2 was assessed after stimulation by lipoteichoic acid (LTA) and streptolysin O (SLO). In addition, TLR2 expression was evaluated after treatment with IFN-gamma and TNF-alpha, and different concentrations of calcium. The expression levels of TLR2 mRNA and protein were studied using RT-PCR and Western blot analysis. RESULTS: Cultured human epidermal keratinocytes constitutively expressed TLR2 and the expression was stimulated by LTA and SLO; in addition, IFN-gamma and TNF-alpha upregulated TLR2 expression. However, the changes in TLR2 expression associated with the calcium concentrations were insignificant. CONCLUSION: TLR2 expression increased with the concentration and duration of bacterial pathogens and this increase was amplified by several cytokines, from activated keratinocytes and other cells.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Streptolysins
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Teichoic Acids
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Bacterial Proteins
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RNA, Messenger
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Keratinocytes
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Lipopolysaccharides
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Blotting, Western
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Calcium
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Cytokines
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Tumor Necrosis Factor-alpha
Limits:
Humans
Language:
En
Journal:
Annals of Dermatology
Year:
2009
Type:
Article