Formulation and Characterization of Antigen-loaded PLGA Nanoparticles for Efficient Cross-priming of the Antigen
Immune Network
;
: 163-168, 2011.
Article
in English
| WPRIM
| ID: wpr-175306
ABSTRACT
BACKGROUND:
Nanoparticles (NPs) prepared from biodegradable polymers, such as poly (D,L-lactic acid-co-glycolic acid) (PLGA), have been studied as vehicles for the delivery of antigens to phagocytes. This paper describes the preparation of antigen-loaded PLGA-NPs for efficient cross-priming.METHODS:
NPs containing a similar amount of ovalbumin (OVA) but different sizes were produced using a micromixer-based W/O/W solvent evaporation procedure, and the efficiency of the NPs to induce the cross-presentation of OVA peptides were examined in dendritic cells (DCs). Cellular uptake and biodistribution studies were performed using fluorescein isothiocyanate (FITC)-loaded NPs in mice.RESULTS:
The NPs in the range of 1.1~1.4microm in size were the most and almost equally efficient in inducing the cross-presentation of OVA peptides via H-2Kb molecules. Cellular uptake and biodistribution studies showed that opsonization of the NPs with mouse IgG greatly increased the percentage of FITC-positive cells in the spleen and lymph nodes. The major cell type of FITC-positive cells in the spleen was macrophages, whereas that of lymph nodes was DCs.CONCLUSION:
These results show that IgG-opsonized PLGA-NPs with a mean size of 1.1microm would be the choice of biodegradable carriers for the targeted-delivery of protein antigens for cross-priming in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovum
/
Peptides
/
Phagocytes
/
Polyglycolic Acid
/
Polymers
/
Spleen
/
Dendritic Cells
/
Immunoglobulin G
/
Ovalbumin
/
Isothiocyanates
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2011
Type:
Article
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