Safety and immunogenicity of recombinant rabies virus (ERAGS) in mice and raccoon dogs
Clinical and Experimental Vaccine Research
; : 159-168, 2016.
Article
in En
| WPRIM
| ID: wpr-176937
Responsible library:
WPRO
ABSTRACT
PURPOSE:
The development of a genetically modified live rabies vaccine applicable to wild raccoon dogs is necessary for the eradication of rabies in Korea. Thus, we constructed a recombinant rabies virus (RABV) called the ERAGS strain, using a reverse genetic system and evaluated its safety and efficacy in mice and its safety and immunogenicity in raccoon dogs. MATERIALS ANDMETHODS:
ERAGS, which has Asn194Ser and Arg333Glu substitutions in the glycoprotein, was constructed using site-directed mutagenesis. Mice were inoculated with the ERAGS strain (either 10(5.0) or 10(7.0) FAID(50)/mL) via intramuscular (IM) or intracranial injections and then challenged with a virulent RABV. Raccoon dogs were administered the ERAGS strain (10(8.0) FAID(50)/mL) either orally or via the IM route and the immunogenicity of the strain was evaluated using fluorescent antibody virus neutralization tests.RESULTS:
The ERAGS strain inoculated into murine neuroblastoma cells reached 10(7.8) FAID(50)/mL at 96-hour post-inoculation. The virus was not pathogenic and induced complete protection from virulent RABV in immunized 4- and 6-week-old mice. Korean raccoon dogs immunized with the ERAGS strain via IM or oral route were also safe from the virus and developed high titer levels (26.4-32.8 IU/mL) of virus-neutralizing antibody (VNA) at 4 weeks post-inoculation.CONCLUSION:
The ERAGS RABV strain was effectively protective against rabies in mice and produced a high VNA titer in raccoon dogs.Key words
Full text:
1
Index:
WPRIM
Main subject:
Rabies
/
Rabies virus
/
Raccoons
/
Neutralization Tests
/
Vaccines
/
Rabies Vaccines
/
Glycoproteins
/
Mutagenesis, Site-Directed
/
Raccoon Dogs
/
Korea
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Clinical and Experimental Vaccine Research
Year:
2016
Type:
Article