Contralateral allodynia and central change in the chronic post-ischemic pain model rats / 대한마취과학회지
Korean Journal of Anesthesiology
; : 419-424, 2009.
Article
in Ko
| WPRIM
| ID: wpr-179767
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Mirror-image allodynia is a mysterious phenomenon that occurs in association with many clinical pain syndromes including complex regional pain syndromes (CRPS). Underlying mechanisms for the development of such pain are still a matter of investigation. Several studies suggest that activation of the N-methyl-D-aspartate (NMDA) receptor is essential for central sensitization as a base for persistent pain. The aim is to assess whether alteration of NMDA receptor expression correlates with the contralateral allodynia in the chronic post-ischemia pain (CPIP) model rats representing CRPS-Type I. METHODS: Application of a tight-fitting tourniquet for a period of 3 hours before reperfusion produced CPIP in male Sprague-Dawley rats. The mechanical paw withdrawal thresholds to von Frey stimuli (using a dynamic plantar aesthesiometer) were measured as pain indicators in ipsilateral and contralateral hindpaws. Phosphorylation of the NMDA receptor 1 subunit (pNR1), assessed with Western blot, was measured in the contralateral L4-6 spinal cord. RESULTS: Ipsilateral and contralateral mechanical allodynia is present at 4 hours after reperfusion, peaked at 3 days, and continued for 7 days after reperfusion. The relative density of pNR1 of CPIP rats significantly decreased in the contralateral L4-6 spinal cord compared to baseline value (P < 0.05). There was significant correlation between paw withdrawal threshold and the relative density of pNR1 (ipsilateral; R2 = 0.75, P < 0.01, contralateral; R2 = 0.60, P < 0.01). CONCLUSIONS: These data suggest that pNR1 is correlated to the contralateral mechanical allodynia in CPIP rats.
Key words
Full text:
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Index:
WPRIM
Main subject:
Phosphorylation
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Prostaglandins E
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Specific Gravity
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Spinal Cord
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Tourniquets
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Reperfusion
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Blotting, Western
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N-Methylaspartate
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Rats, Sprague-Dawley
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Complex Regional Pain Syndromes
Limits:
Animals
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Humans
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Male
Language:
Ko
Journal:
Korean Journal of Anesthesiology
Year:
2009
Type:
Article