Comparison of the 13C-urea breath test and the endoscopic phenol red mucosal pH test in the quantification of Helicobacter pylori infection loading
The Korean Journal of Internal Medicine
; : 134-139, 2008.
Article
in En
| WPRIM
| ID: wpr-181615
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
The 13C-urea breath test (UBT) is a semiquantitative test for measuring Helicobacter pylori infection loading. H. pylori produces ammonia, which elevates the pH of the gastric mucosa and is detectable via endoscopy using a phenol red indicator. We evaluated whether this test could be used to diagnose H. pylori infection and whether phenol red staining was correlated with 13C-UBT results.METHODS:
One hundred and twenty-three patients participated. The UBT was performed after ingestion of a capsule containing urea. A change in 13C-UBT >2 ppt was selected as the cutoff value for diagnosing infection. After spraying evenly with a 0.1% phenol red solution, the pH of the gastric mucosal surface was measured using an antimony electrode through the biopsy channel.RESULTS:
The pH of stained mucosa (6.9+/-0.4) was significantly higher than that of unstained mucosa (1.9+/-0.8; p<0.001), and the H. pylori detection rate confirmed via histology was higher in stained versus unstained mucosa (p<0.01). Extensive mucosal staining resulted in a higher detection rate (p<0.001). The UBT produced results were very similar to those obtained via histological detection in stained mucosa (p<0.001). The extent of staining, expressed as a staining score, was positively correlated with the change in 13C-UBT (r=0.426, p<0.001). A significant correlation was also observed between the histologically determined H. pylori density and 13C-UBT results (r=0.674, p<0.001).CONCLUSIONS:
H. pylori infection elevates gastric mucosal surface pH, and endoscopic phenol red staining may be an alternative method for the diagnosis of H. pylori infection.Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenolsulfonphthalein
/
Urease
/
Breath Tests
/
Endoscopy, Gastrointestinal
/
Helicobacter pylori
/
Helicobacter Infections
/
Clinical Enzyme Tests
/
Esophageal pH Monitoring
/
Gastric Mucosa
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
En
Journal:
The Korean Journal of Internal Medicine
Year:
2008
Type:
Article